calcium pyrophosphate crystals in synovial fluid

#CALCIUM PYROPHOSPHATE CRYSTALS IN SYNOVIAL FLUID MANUAL#

Genetic factors in the pathogenesis of CPPD crystal deposition disease. 2011 Apr 70(4):563-70.įamilial forms of the disease have been reported. European League Against Rheumatism recommendations for calcium pyrophosphate deposition. CPPD typically occurs in older people but can occur in younger people with associated metabolic conditions, such as hyperparathyroidism and haemochromatosis. Diagnostic mimicry in arthritis: patterns of joint involvement associated with calcium pyrophosphate dihydrate crystal deposits.

The chronic form of CPP arthritis mimics osteoarthritis or rheumatoid arthritis and is associated with variable degrees of inflammation. Knees, wrists, shoulders, ankles, elbows, or hands can be affected. Acute CPP crystal arthritis is an acute inflammatory arthritis of one or more joints. Criterion I - Demonstration of calcium pyrophosphate crystal deposition in tissue or synovial fluid by definitive means (eg, characteristic radiographs, diffraction analysis, or chemical. Gordon C, Swan A, Dieppe P (1989) Detection of crystals in synovial fluids by light microscopy: sensitivity and reliability.Calcium pyrophosphate deposition (CPPD) is associated with both acute and chronic arthritis. As a result, diagnostic accuracy is highly user-dependent 5. However, CPP crystal size and weak birefringence makes crystal identification challenging. Petrocelli A, Wong AL, Swezey RL (1998) Identification of pathologic synovial fluid crystals on gram stains. Compensated polarized light microscopy (CPLM) of synovial fluid is the gold standard diagnostic method for CPP crystal-associated arthropathy 4. Selvi E, Manganelli S, Catenaccio M, De Stefano R, Frati E, Cucini S et al (2001) Diff Quik staining method for detection and identification of monosodium urate and calcium pyrophosphate crystals in synovial fluids. McGill NW, Swan A, Dieppe PA (1991) Survival of calcium pyrophosphate crystals in stored synovial fluids. Storage has little influence on the results.

#CALCIUM PYROPHOSPHATE CRYSTALS IN SYNOVIAL FLUID MANUAL#

Salinas M, Rosas J, Iborra J, Manero H, Pascual E (1997) Comparison of manual and automated cell counts in EDTA preserved synovial fluids. Calcium pyrophosphate and monosodium urate crystals in synovial fluid as a cause of pseudoeosinophilia Clin Chem Lab Med. Galvez J, Saiz E, Linares LF, Climent A, Marras C, Pina MF et al (2002) Delayed examination of synovial fluid by ordinary and polarised microscopy to detect and identify crystals. Robier C, Neubauer M, Stettin M, Rainer F (2011) Microscopic examination of stained cytospin preparations is a reliable method for the detection of calcium pyrophosphate crystals in synovial fluid. Kerolus G, Clayburne G, Schumacher HR Jr (1989) Is it mandatory to examine synovial fluids promptly after arthrocentesis? Arthritis Rheum 34(1):118–20 In conclusion, dried cytospin preparations were confirmed as a suitable material for long-time storage and delayed crystal identification. Thus, compared to the examined wet preparations at baseline, there were no false positive or false negative results observed. The crystal content of the initially MSU- and CPPD-positive samples was positively confirmed at any time point of the study, whereas the controls remained crystal-negative during the whole study period. These crystals become more numerous as people age, appearing in nearly half the population older than age 85. After cytocentrifugation for 10 min at 700 rpm in a Shandon Cytospin 4 cytocentrifuge (Thermo Fisher Scientific, Waltham, USA), the sediments were dried on the slides and examined in blinded fashion at any time point by an experienced analyst using polarized microscopy. Pseudogout has been linked to the presence of calcium pyrophosphate dihydrate crystals within the affected joint. For this purpose, we analyzed ten MSU-positive, ten CPPD-positive and 20 crystal-negative SF at baseline (wet preparation), after 24 h, 1 week, 4 weeks, 6 months and 12 months for the occurrence of crystals. Septic arthritis is typically associated with synovial fluid white blood cell counts >20,000 cells/microL, but lower counts may be observed, especially for arthritis due to disseminated gonococcal. WBC: white blood cell MSU: monosodium urate CPPD: calcium pyrophosphate crystal deposition. The aim of this study was to evaluate dried SF cytospin preparations as a suitable medium for long-time storage and delayed crystal analysis. Guide to interpretation of synovial fluid analysis.

Calcium pyrophosphate crystals in synovial fluid